Bio X4 is a weight loss supplement manufactured by a brand called Nucific, which is primarily formulated with probiotics. The brand claims that their product "is more than just a weight management supplement," and also supports gut health.
But does Bio X4 contain research-backed ingredients for weight loss? Does it contain any questionable additive ingredients? How do real users rate and describe its effects? And which retailer sells Bio X4 for the best price?
In this article we'll answer all of these questions and more, as we analyze the ingredients in Bio X4 based on clinical studies to give our take on whether or not this supplement is likely to cause weight loss, and whether or not it's healthy.
We'll also feature unsponsored customer reviews of the brand, and provide a cost comparison to show which retailer sells Bio X4 for the best price.
Bio X4 has a lengthy Supplement Facts label with many active ingredients, so we'll break our ingredient analysis into four sections based on the four different blends in the supplement: Probiotic Blend, Digestive Enzyme Blend, Weight Management Blend, and finally the Craving Control Blend.
Key takeaways:
- Contains a probiotic species clinically shown to cause weight gain
- May support optimal gut health due to probiotics
- We do not currently recommend Bio X4
Probiotic Blend Analysis
The ingredients in Bio X4's Probiotic Blend are shown below:
Lactobacillus acidophilus has been shown to increase weight in both human and animal studies, which makes it a strange choice for a weight loss supplement in our opinion.
A medical review published in the Microbial Pathogenesis journal analyzed data from clinical trials on this probiotic species, and concluded the following: "Lactobacillus acidophilus administration resulted in significant weight gain in humans and in animals."
Bifidobacterium animalis is a probiotic species that may be associated with weight loss.
A 2014 clinical trial found that B. animalis subspecies lactis 420 prevented obesity in animals, but that doesn’t necessarily mean that the generic B. animalis included in Bio X4 will have this effect.
Most clinical trials on probiotic supplementation that we've come across specify probiotic strain, and different probiotic strains within the same species can have totally different biological effects.
Bifidobacterium lactis is clinically shown to support a healthy gut, as we documented in our review of gut support supplement Bioma.
Our verdict: This blend is likely to support gut function but we're unconvinced of its potential weight loss efficacy.
Digestive Enzyme Blend Analysis
The ingredients in Bio X4's Digestive Enzyme Blend are shown below:
A 2016 medical review suggests that digestive enzyme supplementation can be beneficial in the treatment of gastrointestinal diseases like exocrine pancreatic insufficiency and lactose intolerance.
However, we can't find any clinical evidence that digestive enzymes benefit otherwise healthy individuals.
When we initially published this article, Bio X4's product page cited a clinical trial that failed to prove that amylase either caused weight loss or supported gut function.
Since we referenced this, the brand has removed this link and associated marketing claim from its website.
Our verdict: This blend may be beneficial for individuals with gastrointestinal disease, but we can't find any evidence that it supports weight loss or gut function in individuals without gastrointestinal disease.
Weight Management Blend Analysis
The only ingredient in Bio X4's Weight Management Blend is shown below:
Green tea extract is included at a dose of 100 milligrams (mg) standardized to 50% epigallocatechin gallate (EGCG), which equates to 50 mg EGCG per serving.
A 2017 meta-study found that EGCG causes weight loss at a daily dose between 100 mg and 460 mg.
A medical review reported that for a supplement containing green tea extract to be approved for weight management claims by Health Canada, it must have between 126 and 300 mg EGCG.
The use instructions on the Bio X4 website suggest to take the supplement with every meal, so this should be an effective dose for weight loss.
We don't currently recommend supplements containing green tea extract, because this ingredient may cause liver damage in some individuals when taken at high doses, as we documented in our It Works reviews article.
Although we do not consider the dose in this supplement to be high enough to cause liver damage, we err on the side of caution.
Our verdict: This blend is likely to cause weight loss, but we have concerns about the active ingredient choice.
Craving Control Blend Analysis
The only ingredient in Bio X4's Craving Control Blend is shown below:
Slimaluma was shown to cause weight loss in a clinical trial published in the Complementary Therapies in Medicine journal.
However, the dose used was 1,000 mg, or more than 500% of the dose-per-serving in Bio X4.
A more recent medical review analyzed much of the available research on the botanical ingredient in Slimaluma, and concluded that when clinical trials were properly controlled with a placebo, the ingredient was ineffective:
"Appetite parameters showed no significant changes and metabolic parameters did not improve with C. fimbriata supplementation therefore it is unlikely to recommend C. fimbriata as a weight loss supplement and an appetite suppressant."
Our verdict: We're unconvinced that this blend will cause weight loss.
Bio X4 vs. Popular Weight Loss Supps
Here's how Bio X4 compares to other popular weight loss supplements in terms of potential effectiveness and healthiness, in our opinion:
Burn Evolved
Contains one mineral which is clinically shown to cause weight loss, but that mineral is included at nearly 2,000% of the Daily Value (DV).
We consider Bio X4's formulation to be significantly safer for this reason.
Winner: Bio X4
ZipSlim
Contains no active ingredients we could find research backing for to support weight loss, as we documented in our ZipSlim review article.
Also contains inactive ingredients we consider unhealthy.
Winner: Bio X4
Iaso Tea
Contains at least one active ingredient which is clinically shown to cause liver damage.
Winner: Bio X4
Our Clean Weight Loss Picks
Ceylon cinnamon was described as "an effective anti-obesity agent" in a 2022 meta-analysis. The study authors concluded that effects were greater at doses at or over 3 grams daily.
Illuminate Labs Ceylon Cinnamon Extract is our standardized Ceylon cinnamon supplement which is third-party tested to ensure purity and potency.
Dietary fiber was shown in a medical review published in The Journal of Nutrition to cause 16 pounds of weight loss in 6 months when combined with moderate caloric restriction (750 calories per day below baseline).
MBG Organic Fiber Potency+ is our top fiber pick because it's certified organic, provides 7 g of fiber per serving and costs under $1.85 per serving at the time of updating this article.
Both of the products mentioned in this section are free of ingredients that we consider to be unhealthy.
Bio X4 Pros and Cons
Pros:
- Free of unhealthy inactive ingredients
- Effective EGCG dose for weight loss
- Should support gut health due to probiotics
- Stimulant-free
- Free shipping on brand's website
Cons:
- Doesn't appear to be clinically tested
- High doses of green tea extract may be harmful to the liver in some individuals
- Expensive
- Digestive enzyme inclusion is questionable
- Slimaluma inclusion and dosage is questionable
- Includes probiotic species shown to cause weight gain
